Recent advances in hydrophilic interaction liquid interaction chromatography materials for glycopeptide enrichment and glycan separation
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چکیده
منابع مشابه
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phas...
متن کاملSeparation Mechanisms in Hydrophilic Interaction Chromatography
Hydrophilic interaction chromatography (HILIC) is a technique that has become increasingly popular for the separation of polar, hydrophilic, and ionizable compounds, which are diffi cult to separate by reversed phase chromatography (RP) due to their poor retention when RP is used. HILIC typically uses a polar stationary phase such as bare silica or a polar bonded phase, together with an eluent ...
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In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
متن کاملRecent advances in application of hydrophilic interaction chromatography for quantitative bioanalysis.
Hydrophilic interaction chromatography (HILIC) provides a complementary separation mode to RPLC and thus has been gaining increased utilization in LC-MS/MS based quantitative bioanalysis. It has proven to be a powerful tool for separation of polar compounds and has afforded increased selectivity, higher sensitivity, and improved efficiency for quantitation of drug and their metabolites in compl...
متن کاملCharacterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides.
Hydrophilic interaction chromatography (HILIC) is a liquid chromatographic separation mechanism commonly used for polar biological molecules. The use of enhanced-fluidity liquid chromatography (EFLC) with mixtures of methanol/water/carbon dioxide is compared to acetonitrile/water mobile phases for the separation of nucleosides and nucleotides under HILIC conditions. Enhanced-fluidity liquid chr...
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ژورنال
عنوان ژورنال: TrAC Trends in Analytical Chemistry
سال: 2020
ISSN: 0165-9936
DOI: 10.1016/j.trac.2019.06.020